Quirindi's Brooke Saunders and Moree's Ashleigh Walker will make their Super W debuts when the Melbourne Rebels tackle the NSW Waratahs in their season opener on Friday afternoon.
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Saunders has been named to start in the second row and Walker at inside centre.
Rebels head coach Alana Thomas said preparations for the season have gone well.
"We had a really targeted approach to how we ran the preseason with our camp and layout of sessions," Thomas said.
"There was a big focus on both the on and off field culture of the team and I feel this balance has got us in a really good space leading into round one.
"Our trial matches showed this was working and the group has a really good vibe to it."
Thomas believes embracing individual expression and pride in the Melbourne jersey will go a long way towards clinching the club's first win in the Super W competition.
"The focus has been on us as a team and how we want to play and express ourselves on the field.
"It started on Saturday with the cap and jersey presentation."
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"The players have been working hard on the way we will play the game and know that we have a tough opponent in the Waratahs but I think, at times, we get caught up focusing on the opposition and lose your way a touch."
"This group is proud of what has come before us and how they are going to add to that."
With nearly two-thirds of the squad being new to the club, strong leadership will be an important focal point all season, something Thomas believes her side carries in co-captains Melanie Kawa and Meretiana Robinson.
"Our captains have been outstanding," she said.
"They lead from the front; when they speak the players listen.
"The experience Mel brings across a wide playing career will help in the critical moments in the game and you can be assured that she will be in the thick of the action.
"Mere has grown as a leader and this year has support so she can really focus on leading and playing some good rugby."
The match will kick-off at 4.35pm and be followed by the men's clash, which will see Glen Innes' Alex Newsome push into the centres after starting on the wing for the Waratahs' opening two games.